Awareness Hub
CLUE-H Outcomes
Standard Operating Procedures (SOPs) Of the CLUE-H
NextGEM SOPs
- SOP 1: SH-SY5Y cell culture conditions and handling
- SOP 2: Cell cultures exposure to 4G LTE signal at 1950 MHz
- SOP 3: Intracellular ROS measurement by flow cytometer in SH-SY5Y cells
- SOP 4: Apoptosis detection by flow cytometer in SH-SY5Y cells
- SOP 5: Cell cycle progression by flow cytometer in SH-SY5Y cells
- SOP 6: Cell cultures simultaneous exposure to 4G LTE signal at 1950 MHz and WiFi signal at 2450 MHz
- SOP 7: HaCaT cell culture conditions and handling
- SOP 8: Cell cultures exposure to 5G signal at 26.5 GHz
- SOP 9: Cell cultures exposure to UVB radiation
- SOP 10: Intracellular ROS measurement by flow cytometer in HaCaT cells
- SOP 11: Apoptosis detection by flow cytometer in HaCaT cells
- SOP 12: HaCaT cell culture conditions, and handling
- SOP 13: In vitro micronucleus test in human keratinocyte HaCaT cells and 5G RF-EMF exposure
- SOP 14: In vitro comet assay in human keratinocyte HaCaT cells and 5G RF-EMF exposure
- SOP 15: DNA-methylation measurement in HaCaT cells and 5G RF-EMF exposure
- SOP 16: Oxidative stress in HaCaT cells
- SOP 17: TempO-Seq sample preparation
- SOP 18: C. elegans maintenance and handling
- SOP 19: C. elegans exposure to 5G signal at 26.5 GHz
- SOP 20: Health assessment in Caenorhabditis elegans after 5G exposure
- SOP 21: Reproductive toxicity in Caenorhabditis elegans after 5G exposure
- SOP 22: RNA extraction from C. elegans
SEAWave SOPs
- SOP 23: Protocol transcriptomics and DNA methylation assay
- SOP 24: Protocol cell culture and 5G FR2 exposure
- SOP 25: Protocol PNA FISH (Fluorescence In Situ Hybridization)
ETAIN SOPs
- SOP 26: Culture Protocol for HaCaT Keratinocyte Cells
- SOP 27: Primary human keratinocyte culture
- SOP 28: Culture Protocol for EpiDerm Mattek
- SOP 29: Reconstructed epidermis on polycarbonate membrane
- SOP 30: Culture Protocol for skin fibroblasts XP6BE cell line
- SOP 31: Insect Shipping Methods for the ETAIN Project
GOLIAT SOPs
- SOP 32: Plasmids for BRET constructs
- SOP 33: Protocol for Cell Exposure to a 3.5 GHz Electromagnetic Field
- SOP 34: Protocol for Cell Exposure to a 700 MHz Electromagnetic Field
- SOP 35: Proliferation/CFSE Labeling Cytometry SH-SY5Y
- SOP 36: Culture Protocol for Rat Primary Cortical Astrocytes
- SOP 37: Culture Protocol for SH-SY5Y Cells
- SOP 38: Mitosox Red Cytometry Astrocytes
- SOP 39: Mitosox Red Cytometry SH-SY5Y
- SOP 40: Proliferations/CFSE Labeling Cytometry SH-SY5Y
- SOP 41: Reconstructed dermal tissue
- SOP 42: Mitochondrial membrane depolarization in Dermal Sheets
- SOP 43: Mitochondrial Superoxide production in Dermal Sheets
- SOP 44: DNA methylation analysis